Introduction

Bulk RNA-seq Dataset

For exploration of gene and transposable element (TE) expression pattern in hPGCLC differentiation at bulk level, we use RNA-seq dataset from previous publications GSE93126 (Chen et al., 2018) for data visualization. hESC, iMeLC and induced hPGCLC Aggregates of UCLA1 cell line and UCLA2 cell line are used.

Single Cell RNA-seq Dataset

For exploration of gene and transposable element (TE) expression pattern in hPGCLC differentiation at single cell level, we use scRNA-seq dataset from previous publications GSE140021 (Chen et al., 2019) for data visualization. hESC, iMeLC and induced hPGCLC Aggregates (Day 1-4) of UCLA1 cell line and UCLA2 cell line are used. UCLA1 and UCLA2 hESCs were differentiated in duplicate according to the methods of Chen, et al (2019). Human primordial germ cells are specified from lineage primed progenitors. Cell Reports 29: 4568-4582.e5. Displayed are the 10X genomics data acquired from unsorted single cells at each day (d) of differentiation from each replicate (r). The induction of hPGCLCs occurs 24-48 hours after BMP4 exposure.